Figure 1. Whole-cell patch clamp recordings of non-linear capacitive (charge movement) currents in WT and KO FDB fibres.

A and B, representative charge movement current traces elicited by 80 ms depolarizing steps from a holding potential of −80 mV. In all traces dotted line represents the zero baseline. C, zoom in of the ON current of superimposed WT and KO current traces demonstrates suppression of temporally delayed ‘hump’ component (Q_γ) in KO fibres. Typically WT fibres developed a noticeable hump around −20 mV, whereas no distinct second component was seen in KO fibres. Colour-coordinated hash marks on current traces represent integration times to establish ‘Q’ for WT and KO fibres, set to 20, 25, or 30 ms depending on the appearance of a temporally delayed component. A single hash mark implies that the same time to integration was used for both traces.