Figure 6. The Q–V relationship of WT–KO difference component, Q_γ.

A, the average KO recording was subtracted from each individual WT current trace, and the resulting difference trace was integrated to establish Q during the step. The green trace represents the fit to the average difference Q–V, with Boltzmann parameters of Q_max= 12.1 nC μF⁻¹, V_half=−17.7 mV and k= 5, similar to those from the theoretically isolated difference component seen in Fig. 3B. The black trace is the slightly steeper curve generated from averaging the Boltzmann parameters of the individual fits to the difference Q–Vs. B, average difference (WT – KO) Q–V plotted in scale with WT and KO Q–V relationships from Fig. 3B. C, normalized Q–V relationships of fits from panel B.