Figure 7. Rescue of Q_γ with purified S100A1.

A, KO fibres dialysed, via the patch pipette, with 10 μm Alexa-488-S100A1 show restoration of Q–V relationship to WT levels. S100A1 protein concentration in KO fibres was estimated using confocal fluorescence microscopy and averaged 2.8 ± 0.21 μm at the time of recordings. B, zoom in of ON current traces from KO AVG and representative KO + S100A1 fibre show that kinetic properties of charge movement currents can be restored in KO fibres dialysed with S100A1, specifically the temporally delayed Q_γ component.