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Protein synthesis of gp130 is suppressed by AG490. (A) Cells were pretreated with cycloheximide (CHX, 10 µM) for 4 hrs, and then washed and reincubated in normal growth medium with or without AG490 (50 µM) for the indicated times. W1; reincubation in normal medium for 1 hr after 4 hrs of CHX treatment. (B) Quantitative results showing the change in gp130 levels following CHX treatment.
