Fig. 2.

Role of ROS on HNE-induced CD36 expression in J774A.1 macrophages. Macrophages were treated with 10 µM HNE for the indicated time in the absence or presence of N-acetylcysteine (Nac, 5 mM). The levels of CD36 mRNA and protein were determined by RT-PCR (A) and Western blot analysis (B), respectively. Each fig is the representative of 4 independent experiments. Data was presented as mean±SE from 5 independent experiments. ^**p<0.01 vs. control; ^##p<0.01 vs. veh (vehicle).