Fig. 3.

The effect of rotenone on the phosphorylation of JNK and P38 in SH-SY5Y cells. (A) The effect of rotenone on the phosphorylation of JNK and P38 by Western blot analysis. Cells were treated with 10 µM rotenone for 120 min. (B) Western blot to determine the effect of naringin on rotenone-induced phosphorylation of JNK and P38 in SH-SY5Y cells. The cells were pretreated with 10 µM rotenone for 120 min, with 10 µM naringin added 4 h prior to rotenone treatment. The cells were harvested for the phosphorylation assay at 30 and 120 min after rotenone treatment. β-Actin was used as an internal standard protein. Three independent experiments were performed for this assay.