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. 2009 Jun 30;13(3):161–168. doi: 10.4196/kjpp.2009.13.3.161

Fig. 2.

Fig. 2

IL-6 induces serine phosphorylation of STAT3 in Schwann cells. RT4 schwannoma cells (A~D) and primary SCs (E, F) were serum starved overnight and then treated with IL-6 (50 ng/ml) for the indicated times. Total cell lysates were subjected to Western blot analysis using antibodies specific for phosphorylated proteins. (A, B) RT4 cells were treated with IL-6 for 15 min, and the cellular lysates were analyzed using the indicated antibodies. (B) A quantitative analysis of tyrosine phosphorylated STAT3 in result (A). (C, D) RT4 cells were treated with IL-6 for the indicated time in the presence or absence of U0126. (D) A quantitative analysis of serine phosphorylated STAT3 in result (C). (E, F) Primary SCs were treated with IL-6 for 15 min, and the total lysates were analyzed by Western blot analysis using the indicated antibodies. (F) A quantitative analysis of phosphorylated STAT3 in result (E).