Fig. 4.

Enzyme immobilization pathways using the glycidyl methacrylate-based monolithic supports: (A) direct immobilization via epoxide functionality; (B) immobilization comprising aminolysis with a diamine, activation with glutaraldehyde, reaction with the enzyme, followed by hydrogenation of the aldimine bond with cyanoborohydride; (C) immobilization comprising acid-catalyzed hydrolysis to a diol, periodate oxidation to aldehyde, and reaction with the enzyme in presence of cyanoborohydride; (D) immobilization via acid-catalyzed hydrolysis to a diol, activation of hydroxyl groups with carbonyldiimidazole, followed by reaction with an enzyme.