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Photomicrograph depicting representative examples of beta-endorphin-ir in the PAGl/vl in adult rats following neonatal treatment. (A) handled, 4×, (B) handled, 20×; (C) 1% carrageenan, 20×. (D) Neonatal CGN resulted in significantly increased beta-endorphin-ir in the lateral/ventrolateral regions of the mid and caudal PAG compared to control animals. N = 6–8 rats per group/per sex. * denotes significant main effect of treatment. Error bars represent SEM.
