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. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: FEBS Lett. 2009 Sep 12;583(19):3127–3132. doi: 10.1016/j.febslet.2009.09.013

Figure 3.

Figure 3

CtBPs attenuated KLF4-mediated transcriptional activation. (A): A diagram of an artificial pGL5-Luciferase reporter system was shown, where KLF4 is recruited to the proximal promoter region to activate transcription. (B): Overexpression of CtBP1 and CtBP2 significantly reduced KLF4-mediated transcriptional activation. Transient co-transfections with pGL5 reporter construct and pM vector, pM/KLF4, pM/KLF4 and pFLAG-CtBP1 and pFLAG-CtBP2 with different combination were performed in HCT116 cells, followed by dual luciferase assay as described in Materials and Methods. Means ± SD for 3 independent experiments were shown.