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. Author manuscript; available in PMC: 2010 Oct 6.
Published in final edited form as: FEBS Lett. 2009 Sep 12;583(19):3127–3132. doi: 10.1016/j.febslet.2009.09.013

Figure 4.

Figure 4

CtBPs attenuated KLF4-mediated IAP and KRT4 reporter activation. Similar to Fig 3B except IAP promoter reporter (A) or KRT4 reporter (B) construct was used instead of pGL5 reporter. C. Mutations in CtBP binding motif in KLF4 abolished interaction between KLF4 and CtBP1. Wild type (pMyc-KLF4) and mutant KLF4 (pMyc-KLF4M) that harbors mutations in CtBP binding motif were cotransfected with pFLAG-CtBP1 and co-immunoprecipatation assays were performed to examine the interaction between KLF4 and CtBP1 as described in Figure 1B.