Figure 6. “Jump start” experiment scheme.

The tested strain is grown in the presence of 5 ng ml⁻¹ of synthetic SilCR to late log phase (OD₆₀₀ = 1). Then, the supernatant of the tested culture is collected, diluted 10 or 3-fold and added to the “reporter strain” JS95 or N9 (respectively), harboring pP₄-gfp. After incubation for 2 additional hours at 37°C, the relative fluorescence of the “reporter strain” is determined as described in Figure 2.