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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neurochem. 2009 May 19;110(2):756–764. doi: 10.1111/j.1471-4159.2009.06184.x

Fig. 6. Evidence that NE and cAMP control Mbnl2 mRNA and protein.

Fig. 6

A. Mbnl2 mRNA: Cultured pineal glands were pre-incubated with 3 μM KT5720 alone for 1 hr; NE (1 μM) was then added and incubation was continued for 6 hours. Each lane was loaded 8 μg of total RNA from pools of three or four glands. Northern blots were probed with a rat Mbnl2 probe. To normalize for RNA loading, the blot was reprobed for 18S rRNA. Results are presented as the mean ± SE of three replicates. *, p < 0.01 vs. NE group. B. Mbnl2 protein: Using pineal glands from the same experiment shown in panel A, each lane was loaded with 60 μg protein from a pineal extract. Immunodetection was performed with an anti-Mbnl2 serum at a dilution of 1:10,000. Results are presented as the mean ± SE of three replicates. *, p < 0.01 vs. NE group. C. Pineal glands were cultured for 6 hours with 500 μM 8-Br cAMP (8-Br), 500 μM dibutyryl cAMP (DB) or 1 μM NE; control (CONT). *, p < 0.01 vs. CONT group. For further technical details see 5.A. and the Materials and Methods section.