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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Neurochem. 2009 May 19;110(2):756–764. doi: 10.1111/j.1471-4159.2009.06184.x

Fig. 7. NE-induced elevation in Mbnl2 mRNA requires transcription but not translation.

Fig. 7

Pineal glands were treated with 30 μg/ml actinomycin D (ACTD) or 50 μg/ml puromycin (PURO) for 1 hour and during the subsequent 6 hours treatment with 1 μM NE. Each lane was loaded 8 μg total RNA obtained from a pool of four pineal glands. After hybridization with the Mbnl2 probe, the blot was stripped and reprobed with an 18S rRNA probes as a control. Results were confirmed in three independent experiments. The values are mean ± SE of three replicates. For further details see the Materials and Methods section.