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. Author manuscript; available in PMC: 2010 Oct 28.
Published in final edited form as: Neuroreport. 2009 Oct 28;20(16):1429–1433. doi: 10.1097/WNR.0b013e3283311db6

Fig. 2.

Fig. 2

Stimulation-induced arc expression does not require NMDA receptors if action potentials are maintained. To insure that action potentials were maintained during NMDA receptor antagonist exposure (50 μM D-APV), synaptic stimulation was delivered in a theta burst pattern of stimulation (TBS) with bicuculline [7]. Hippocampal CA1 mini-slices were sampled 15 minutes after stimulation. mRNA was purified from pooled slices, cDNA prepared and real-time quantitative PCR performed. Plotted are data from 4 separate determinations. Stimulated arc levels in the APV-treated slices are not significantly different from the stimulated levels in slices without the drug.