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. Author manuscript; available in PMC: 2009 Oct 26.
Published in final edited form as: Cancer Res. 2008 Sep 15;68(18):7278–7282. doi: 10.1158/0008-5472.CAN-08-1842

Figure 3.

Figure 3

Figure 3

Figure 3

Influence of wound fluid and wounding on tumor cell proliferation. A. Wound fluid increases tumor cell proliferation in vitro. Cells were stimulated with 3% wound fluid or serum or plasma, or were serum starved (0%) for 2 days. Cell numbers were counted and normalized to the average cell number of serum treated cells. ANOVA / Dunnet's Multiple Comparison Test. Data are presented as mean±SEM. B. Wound fluid generated from BALB/c mice but not wound fluid generated from nude mice (BALB/c nu/nu) promotes proliferation of 4T1 cells in vitro. The effect of wound fluid not only depended on the mouse strain, but also on the age of the wound fluid; wound fluid harvested 6 days to 9 days after wounding was most effective in stimulating tumor cell proliferation. ANOVA / Dunnet's Multiple Comparison Test. Data are presented as mean±SEM. C. Wounding increases tumor cell proliferation in-vivo. Mitotic tumor cells were counted in H&E stained sections of tumors obtained from wounded and unwounded animals 23 days after tumor cell inoculation. Student's t-test.