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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Protein Expr Purif. 2009 Jun 30;68(1):34–41. doi: 10.1016/j.pep.2009.06.018

Fig. 1.

Fig. 1

Wild type and C1C5 RANTES construct design and protein secretion. (A) Schematic representation of pcDNA3.1+ and p1063 constructs for wt and C1C5 RANTES expression in CHO cells and L. jensenii, respectively. (B) Reducing and non-reducing Western blot analysis of secreted RANTES variants using anti-human RANTES Abs. st, RANTES standard from E. coli; nt, supernatants from non-transfected CHO cells or non-transformed L. jensenii; and wt and C1C5, wt and C1C5 RANTES-containing supernatants, respectively.