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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Protein Expr Purif. 2009 Jun 30;68(1):34–41. doi: 10.1016/j.pep.2009.06.018

Fig. 2.

Fig. 2

Anti-HIV-1 activity of secreted wt and C1C5 RANTES. Inhibition of HIV-1BaL infection in PM1 cells was measured by the p24-based assay after 4 days of infection. (A) (circles) E. coli-expressed RANTES standard (ID50% = 0.8 nM) and (triangles) CHO cell-secreted wt RANTES (ID50% =0.63 nM). Values are the mean ± SD of two independent experiments performed in triplicate. (B) (white columns) E. coli-expressed RANTES standard; (dotted columns) partially purified wt RANTES from L. jensenii; and (black columns) partially purified C1C5 RANTES from L. jensenii. Values are the mean ± SEM of one experiment performed in triplicate, representative of two experiments yielding similar results.