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. Author manuscript; available in PMC: 2010 Aug 11.
Published in final edited form as: Biochemistry. 2009 Aug 11;48(31):7441–7447. doi: 10.1021/bi900433y

Figure 4. PKR binding and activation in vitro by domain 3 aspartic acid point mutants.

Figure 4

(A) S246D or S287D each activates PKR. The effects of 1, 10, and 100 nM concentrations of purified bacterially-expressed MBP-3 Asp mutants were tested on PKR activation. wt MBP-3 was the positive control. PKR levels were analyzed by Western blotting using anti-PKR antibody. (B) S246D or S287D each binds to PKR. Procedures are described in Figure 3. PhosphorImager analysis was done to quantify the amount of PKR bound. The amount of PKR binding by wt MBP-3 was considered 100%, and the values for other proteins are presented as percentages of that value.