TABLE 1.

Quantitation of PKR binding, activation, and cell surviva

Mutations	Bindinga	Activationb	Cell Survivalc
Wt	100	100	5
Q243A	9	0	100
S246A	0	0	100
D260A	63	26	90
D262A	2	8	93
S265A	0	7	95
Q271A	0	0	95
S279A	23	28	88
S287A	59	0	88
G288A	0	9	93
C291A	0	0	95
S246D	100	96	2
S287D	95	98	2
S246D/S287D	+d	114	7
S246D/S287A	85	0	ND

^aPhosphorImager analysis was done to quantify bands in Fig 3

^b10nM concentration lanes from Fig 2, Fig 4, and Fig 5. Levels of Wt domain 3 are considered as 100% and the values for the mutants are presented as percentages of that value; these experiments were done with MBP-3.

^cStressed cells, from Peters et al., 2006.

^dsee Fig 6. ND=not determined.