TABLE 1.
Quantitation of PKR binding, activation, and cell surviva
Mutations | Bindinga | Activationb | Cell Survivalc |
---|---|---|---|
Wt | 100 | 100 | 5 |
Q243A | 9 | 0 | 100 |
S246A | 0 | 0 | 100 |
D260A | 63 | 26 | 90 |
D262A | 2 | 8 | 93 |
S265A | 0 | 7 | 95 |
Q271A | 0 | 0 | 95 |
S279A | 23 | 28 | 88 |
S287A | 59 | 0 | 88 |
G288A | 0 | 9 | 93 |
C291A | 0 | 0 | 95 |
S246D | 100 | 96 | 2 |
S287D | 95 | 98 | 2 |
S246D/S287D | +d | 114 | 7 |
S246D/S287A | 85 | 0 | ND |
PhosphorImager analysis was done to quantify bands in Fig 3
10nM concentration lanes from Fig 2, Fig 4, and Fig 5. Levels of Wt domain 3 are considered as 100% and the values for the mutants are presented as percentages of that value; these experiments were done with MBP-3.
Stressed cells, from Peters et al., 2006.
see Fig 6. ND=not determined.