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. Author manuscript; available in PMC: 2010 Aug 11.
Published in final edited form as: Biochemistry. 2009 Aug 11;48(31):7441–7447. doi: 10.1021/bi900433y

TABLE 1.

Quantitation of PKR binding, activation, and cell surviva

Mutations Bindinga Activationb Cell Survivalc
Wt 100 100 5
Q243A 9 0 100
S246A 0 0 100
D260A 63 26 90
D262A 2 8 93
S265A 0 7 95
Q271A 0 0 95
S279A 23 28 88
S287A 59 0 88
G288A 0 9 93
C291A 0 0 95
S246D 100 96 2
S287D 95 98 2
S246D/S287D +d 114 7
S246D/S287A 85 0 ND
a

PhosphorImager analysis was done to quantify bands in Fig 3

b

10nM concentration lanes from Fig 2, Fig 4, and Fig 5. Levels of Wt domain 3 are considered as 100% and the values for the mutants are presented as percentages of that value; these experiments were done with MBP-3.

c

Stressed cells, from Peters et al., 2006.

d

see Fig 6. ND=not determined.

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