Abstract
After a prototrophic strain of Staphylococcus aureus had been exposed to diethyl sulfate, 28 isoleucine- and isoleucine-valine-dependent mutants (ilv mutants) were isolated. On the basis of auxanography, their ability to accumulate intermediates of isoleucine and valine biosynthesis, and intergeneric syntrophism with ilv mutants of Salmonella typhimurium, all mutants were placed into four groups, each of which corresponded to a presumed enzymatic deficiency, as follows: group A, deficient in l-threonine deaminase; group B, deficient in the condensing enzyme; group C, deficient in reductoisomerase; group D, deficient in α-β-dihydroxy acid dehydrase. No mutants blocked in the terminal (transaminase) reactions were isolated. Transduction analyses (best-fit, ratio, and complementation tests) with the use of phage 83 established that the linear arrangement of the structural genes is identical with the order of participation of their enzymes in isoleucine and valine biosynthesis, and that these genes comprise a single linkage group which can exist on a single donor fragment during transduction.
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Selected References
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- Armstrong F B, Wagner R P. Mutants of Salmonella Deficient in Reductoisomerase. Genetics. 1962 Nov;47(11):1581–1593. doi: 10.1093/genetics/47.11.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KLOOS W. E., PATTEE P. A. A BIOCHEMICAL CHARACTERIZATION OF HISTIDINE-DEPENDENT MUTANTS OF STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1965 May;39:185–194. doi: 10.1099/00221287-39-2-185. [DOI] [PubMed] [Google Scholar]
- KLOSS W. E., PATTEE P. A. TRANSDUCTION ANALYSIS OF THE HISTIDINE REGION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1965 May;39:195–207. doi: 10.1099/00221287-39-2-195. [DOI] [PubMed] [Google Scholar]
- PATTEE P. A., BALDWIN J. N. Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus. J Bacteriol. 1962 Nov;84:1049–1055. doi: 10.1128/jb.84.5.1049-1055.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PEARCE L. E., LOUTIT J. S. BIOCHEMICAL AND GENETIC GROUPING OF ISOLEUCINE-VALINE MUTANTS OF PSEUDOMONAS AERUGINOSA. J Bacteriol. 1965 Jan;89:58–63. doi: 10.1128/jb.89.1.58-63.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roth J. R., Sanderson K. E. Orientation of the isoleucine-valine genes in the Salmonella typhimurium linkage map. Genetics. 1966 May;53(5):971–976. doi: 10.1093/genetics/53.5.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WAGNER R. P., BERGQUIST A. Synthesis of valine and isoleucine in the presence of a particulate cell fraction of Neurospora. Proc Natl Acad Sci U S A. 1963 Jun;49:892–897. doi: 10.1073/pnas.49.6.892. [DOI] [PMC free article] [PubMed] [Google Scholar]