Table 1.
Additionsb | PalH Activityc |
Control (PalH only) | NDAd |
+ NAD+ | 0.92 |
+ Mn2+ | NDA |
+ DTT | NDA |
+ EDTA | NDA |
+ NAD+ + Mn2+ | 1.27 |
+ NAD+ + DTT | 0.60 |
+ NAD+ + EDTA | NDA |
+ NAD+ + Mn2+ + DTT | 6.09 |
+ NAD+ + Mn2+ + DTT + EDTA | NDA |
PalH used in these experiments had previously been dialyzed for 18 h against 25 mM Tris–HCl (pH 7.5) buffer.
Additions to the basal 2-ml discontinuous assay were as described in Materials and Methods.
PalH activity expressed as μmol pNPαGlc hydrolyzed mg protein−1 min−1. Values are the mean of two separate assays.
NDA, No detectable PalH activity.