Table 1.

Thermodynamic Parameters for DNA Formation at 20 °C.^a

OL	sequence	NaClb	T Mc	ΔG°de	ΔH° e	TΔS°e	ΔnNa+f	Δnwf	ΔΔG vs OL-1 or -5
1	5’-GAGAGCGCTCTC	10	48.7	−6.9	−78.2	−71.3	−3.35 ± 0.17	−41 ± 3	-
		100	66.1	−12.b	−92.0	−79.b	−3.61 +0.18	−43 + 4
2	5’-GAGA-c7G-CGCTCTC	10	44.7	−4.4	−56.3	−51.9	−2.14 ± 0.11	−25 ± 2	2.5
		100	62.0	−8.4	−67.2	−58.8	−2.31 ± 0.12	−27 ± 2	4.1
3	5’-GAGA-1-CGCTCTC	10	52.0	−9.1	−92.9	−83.8	−2.86 ± 0.14	−38 ± 4	−2.2
		100	67.3	−13.9	−99.7	−85.9	−2.90 ± 0.15	−36 ± 3	−1.4
4	5’-GAGA-2-CGCTCTC	10	47.2	−4.6	−54.b	−49.9	−1.63 ± 0.10	−18 ± 2	2.3
		100	63.9	−7.6	−58.2	−50.6	−1.52 ± 0.10	−17 ± 2	4.9
5	5’-CGCGTTTTCGCG	10	68.4	−4.4	−31.0	−26.6	−0.26 ± 0.02	−18 ± 2	-
6	5’-CGCGTTTTC-c7G-CG	10	63.7	−3.b	−27.0	−23.5	−0.21 ± 0.02	−15 ± 2	0.9
7	5’-CGCGTTTTC-1-CG	10	63.0	−4.0	−31.3	−27.3	0	−14 ± 2	0.4
8	5’-GGG-1-TTTTCGCG	10	71.3	−4.3	−28.9	−24.6	0	−16 ± 2	0.1
9	5’-CGC-1-TTTTC-1-CG	10	65.6	−3.6	−27.0	−23.4	0	−13 ± 1	0.8

^aParameters are measured from UV (T_M) and DSC melting curves in 10 mM sodium phosphate buffer (pH 7.0). The observed standard deviations are:T_M (± 0.7), ΔH_cal (± 3%), ΔG°₂₀ (± 5%), TΔS_cal (± 3%).

^bSalt concentration in mM.

^c°C.

^dDetermined at 20 °C.

^ekcal/mol.

^fper mol DNA