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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7888–7896. doi: 10.4049/jimmunol.0800891

Figure 3.

Figure 3

Combination of stress and allergen challenge attenuated the time-dependent inhibitory effect of corticosterone on splenocytes. Spleens were harvested before (0h), 24 and 48h after Af challenge. Splenocytes were cultured in the presence of LPS (1 µg/ml) and 5 µM of corticosterone for 48 hours. Cell viability (top left panel) was determined using a colorimetric assay. Cytokine, chemokine and immunoglobulin levels in the supernatant were measured by SearchLight technology. Data are presented as the percentage of cell viability or cytokine, chemokine and immunoglobulin concentration of control values measured in the absence of corticosterone, marked by dashed lines (Mean±SEM of n=5–8 per group).