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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):7888–7896. doi: 10.4049/jimmunol.0800891

Figure 4.

Figure 4

Combination of stress and allergen challenge abolished the dose-dependent inhibitory effects of corticosterone on LPS-stimulated splenocytes. Spleens were harvested from naïve (open squares), or SDR controls (grey squares) and from Af−sensitized mice 24 h after Af challenge (Af, grey triangles, Af+SDR, black triangles). Splenocytes were cultured in the presence of LPS (1 µg/ml) and and 0, 0.01, 0.05, 0.1, 0.5 or 5 µM of corticosterone for 48 hours. Cell viability (top left panel) was determined using a colorimetric assay. Cytokine, chemokine and immunoglobulin levels in the supernatant were measured by SearchLight technology in each groups. Data are presented as the percentage of cell viability or cytokine, chemokine and immunoglobulin concentration of control values measured in the absence of corticosterone, marked by dashed lines (Mean±SEM of n=5–8 per group).