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. Author manuscript; available in PMC: 2010 Apr 30.

Published in final edited form as: J Phys Chem A. 2009 Apr 30;113(17):5251–5263. doi: 10.1021/jp8082908

FIG. 12 — Contours of constant photon number as labeled on the figure that give (𝕊, 𝕆) equivalent to 95% likelihood of distinguishing two dyes (or states) with lifetimes τ₁ and τ₂. The contours above the diagonal include the effects of a w=1 ns Gaussian instrument response whereas those below the diagonal include a w=100 ps Gaussian IRF. The green zone on the plot shows the ideal region for single molecule measurements.

Inline graphic — Contours of constant photon number as labeled on the figure that give (𝕊, 𝕆) equivalent to 95% likelihood of distinguishing two dyes (or states) with lifetimes τ₁ and τ₂. The contours above the diagonal include the effects of a w=1 ns Gaussian instrument response whereas those below the diagonal include a w=100 ps Gaussian IRF. The green zone on the plot shows the ideal region for single molecule measurements.