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. Author manuscript; available in PMC: 2010 Apr 30.

Published in final edited form as: J Phys Chem A. 2009 Apr 30;113(17):5251–5263. doi: 10.1021/jp8082908

FIG. 16 — Contours of constant photon number as labeled on the figure that give (𝕊, 𝕆) equivalent to 95% likelihood of distinguishing two states at FRET distances of R₁/R₀ and (R₁ + δR)/R₀. The left panel shows the contribution from donor photons, the center panel shows the contribution from acceptor photons, and the right panel shows contributions from both. Experimental parameters are the same as in Fig. 15

Inline graphic — Contours of constant photon number as labeled on the figure that give (𝕊, 𝕆) equivalent to 95% likelihood of distinguishing two states at FRET distances of R₁/R₀ and (R₁ + δR)/R₀. The left panel shows the contribution from donor photons, the center panel shows the contribution from acceptor photons, and the right panel shows contributions from both. Experimental parameters are the same as in Fig. 15