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Capture by g5p (A) and NaPTA (B) of PrP from PSPr and sCJDMM1 (sCJD). Probing with 3F4 or 1E4 after stripping. The same ladder of PK-resistant PrP as in Fig 4D is detectable in PSPr preparations after heavy loading of the gel. S1: Supernatant of brain homogenate obtained after low speed centrifugation (1,000 g for 10 min).
