Skip to main content
. Author manuscript; available in PMC: 2009 Oct 26.
Published in final edited form as: Am J Physiol Endocrinol Metab. 2007 Oct 9;293(6):E1552–E1563. doi: 10.1152/ajpendo.00134.2007

Fig 4. Total and pS293 phosphorylated BCKD E1α and BCKD kinase immunoreactivity in liver from Zucker rats.

Fig 4

Fig 4

Fig 4

Fig 4

Liver lysates made from frozen lean (L) and fa/fa Fatty Zucker (F) rat liver powders, were equalized for protein content, separated by SDS-PAGE and transferred to PVDF. Panel A shows representative immunoblots of BCKD E1α (liver does not express BCATm), pS293 E1α and BCKD kinase from random fed lean (L) and ob/ob (O) mice. Thus the other panels show the densitometric analysis of these blots for BCKD E1α (Panel B), pS293 E1α/BCKD E1α ratio (Panel C) and BCKD kinase (Panel D). An ANOVA was first used to compare the lean and obese states as well as across the fed and fasted states followed by a Student-Newman-Keuls Multiple Comparisons post test. Asterisks indicate where statistical differences between the fatty and lean condition in the corresponding nutritional state was observed (** p < 0.01, *** p < 0.001). No statistical differences were detected between expression levels of any of the antigens when random fed and fasted states were compared. Western blotting for β-Actin was used as a loading control. The densitometry values were 6270 ± 703; 6198 ± 255; 6297 ± 20 and 6786 ± 599 for fed lean, fed obese, fasted lean and fasted obese rat liver, respectively (p>0.05).