Fig 7. Plasma BCAA concentrations in overnight fasted morbidly obese human subjects before and after gastric bypass surgery.
Plasma was collected between 9 and 12 AM after an overnight fast after induction of general anesthesia from subjects described in table 4 before Roux-en-Y gastric bypass surgery and later when subjects returned for a second surgery. Plasma was stored at −80°C until assay for total BCAA concentration was conducted as in Fig. 1 using an enzymatic assay. **p < 0.01 before versus after surgery.