Figure 4.

Viral LMP2A is a potential target of miR-BART22. (A) Base complementarity suggests that the putative miR-BART22 target site is in the 3′UTR of LMP2A (AJ507799, 5545:5568; upper). The wild-type (LMP2A-WT), truncated (LMP2A-M1) and mutated (LMP2A-M2 and -M3) LMP2A sequences are shown. The seed-binding region is underlined and the base substitutions a remarked in red (lower). (B) The sequences of miRNA mimics are displayed. The base substitutions made to restore seed mutant complementarity are labeled in red (miR-M2-BART22 and miR-M3-BART22). The relative firefly luciferase activity was normalized to the renilla luciferase control, and results were taken from at least three independent experiments. Data shown are the mean ± SD. Statistical analysis by Student's t test was used. **P < .001.