Figure 6.

Suppression of LMP2A protein expression by miR-BART22. (A) Western blot of LMP2A and EGFP in 293FT cells transiently cotransfected with 0.1 µg per well pEGFP-C1 plasmid in six-well plate format, the amount of expression plasmids as indicated (lanes 3–6), and 2 µg of total DNA made up with pcDNA3.1 as carrier. LMP2A expression level was normalized to EGFP. miR-BART22 and LMP2A RNA expression levels in the same sample were analyzed by QRTPCR and are shown relative to lane 3. (B) The representative Western blot results indicate that miR-BART22 represses LMP2A expression in HEK293-LMP2A stable clones. The stable clones transfected with the siRNA control (siCtl) and LMP2A specified siRNA (siLMP2A) were included as controls. The LMP2A expression level was normalized to actin and the level relative to mock transfection (set at 1) was calculated. LMP2A mRNA expression in the same sample was assayed by QRT-PCR.