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. 2009 Nov;11(11):1194–1207. doi: 10.1593/neo.09902

Figure 6.

Figure 6

Western blot analysis of the 26S proteasome in the three radioresistant derivatives in comparison with parental cell lines. Each band was quantitated by densitometry using Quantity One software (Bio-Rad) and normalized to a GAPDH loading control. The 26S proteasome antibody (ab21165; Abcam), which recognizes the 20S component of the 26S proteasome, produced the expected band size of approximately 32 kDa. A significant decrease in the expression of the 26S proteasome was observed in all radioresistant derivatives in comparison with parental cell lines: MCF7RR, 7.7-fold decrease; MDA-MB-231RR, 45.1-fold decrease; T47DRR, 6.3-fold decrease.