Table 2.
Proteomics Data, Ranked by Mowse Score, for All Assigned Proteins Associated with In Vitro Radioresistance in MCF7RR, T47DRR, and MDA-MB-231RR Cells Identified Using 2DE in Combination with MALDI-TOF or MALDI-TOF/TOF MS.
| Protein | NCBI Accession | Gene Name | Known/Proposed Function | RR Cell Line | pH Gradient | Expression Level | Mowse Score |
| Heat shock 90-kDa protein 1 beta*,† | 34304590 | HSP90AB1 | Protein folding/degradation | T47D | 4–7 | ▴ | 787 |
| Eukaryotic translation initiation factor 4A, isoform 1*,‡ | 54696624 | EIF4A1 | Protein biosynthesis | T47D | 4–7 | ▴ | 725 |
| Ubiquitin activating enzyme E1*,†,‡ | 35830 | UBA1 | Ubiquitin cycle | T47D | 4–7 | ▴ | 492 |
| α-Tubulin* | 37492 | TUBA1A | Structural | T47D | 4–7 | ▾ | 473 |
| Glutaminyl-tRNA synthetase*,† | 4826960 | QARS | Protein biosynthesis | MDA | 7–10 | ▴ | 309 |
| TRAP1*,† | 3273383 | TRAP1/HSP75 | Protein folding/degradation | T47D | 4–7 | ▴ | 302 |
| G elongation factor, mitochondrial 1* | 18390331 | GFM1 | Protein biosynthesis | T47D | 4–7 | ▴ | 177 |
| Triosephosphate isomerase 1*,† | 17389815 | TPI1 | Metabolism | MDA | 7–10 | ▾ | 177 |
| Lymphocyte cytosolic protein 1 (l-plastin)† | 14043359 | LCP1 | Structural | MCF7 | 4–7 | ▾ | 155 |
| Mitochondrial malate dehydrogenase precursor‡ | 21735621 | MDH2 | Metabolism | T47D | 7–10 | ▴ | 149 |
| Hypothetical (PGM2) | 12052930 | PGM2 | Metabolism | MDA | 4–7 | ▾ | 149 |
| HSPC263 | 6841176 | OTUB1 | Ubiquitin cycle | T47D | 4–7 | ▴ | 148 |
| Vinculin isoform VCL | 4507877 | VCL | Cell adhesion | T47D | 4–7 | ▴ | 144 |
| Actinin, alpha 4 | 12025678 | ACTN4 | Structural | MCF7 | 4–7 | ▴ | 133 |
| Eukaryotic translation elongation factor 2 | 4503483 | EEF2 | Protein biosynthesis | T47D | 4–7 | ▴ | 133 |
| Solute carrier family 25, member 24 | 46249805 | SLC25A24 | Transport | MDA | 4–7 | ▴ | 131 |
| Fructose-bisphosphate aldolase A† | 4557305 | ALDOA | Metabolism | T47D | 7–10 | ▴ | 125 |
| Proteasome subunit, alpha type, 7*,† | 30583169 | PSMA7 | Protein degradation | MDA | 7–10 | ▾ | 124 |
| Voltage-dependent anion channel 1 | 4507879 | VDAC1 | Transport | T47D | 7–10 | ▴ | 121 |
| Aspartyl-tRNA synthetase† | 179102 | DARS | Protein biosynthesis | T47D | 4–7 | ▴ | 118 |
| Aspartyl-tRNA synthetase† | 62896511 | MDA | ▾ | 95 | |||
| Glutathione S-transferase M3†,§ | 14250650 | GSTM3 | Metabolism | MCF7 | 4–7 | ▾ | 118 |
| Keratin 17† | 48735384 | KRT17 | Structural | MCF7 | 4–7 | ▾ | 117 |
| Pyrophosphatase 1 | 85397510 | PPA1 | Metabolism | MCF7 | 4–7 | ▾ | 115 |
| Moesin† | 4505257 | MSN | Structural | MDA | 4–7 | ▾ | 108 |
| Leucine-rich PPR motif-containing protein | 31621305 | LRPPRC | Transport | MCF7 | 4–7 | ▴ | 108 |
| N-Acetyltransferase ARD1 | 10835057 | ARD1A | Protein modification | MCF7 | 4–7 | ▾ | 105 |
| Proteasome activator subunit 2 (PA28 beta)§ | 13325110 | PSME2 | Protein degradation | MCF7 | 4–7 | ▾ | 105 |
| Glyoxalase domain containing 4 | 16198390 | GLOD4 | Unknown | MCF7 | 4–7 | ▾ | 101 |
| EPB41 | 24657823 | EPB41 | Structural | MDA | 4–7 | ▾ | 101 |
| Proteasome subunit, alpha type, 2* | 51094758 | PSMA2 | Protein degradation | MDA | 7–10 | ▾ | 101 |
| Spermidine synthase | 63253298 | SRM | Spermidine biosynthesis | MDA | 4–7 | ▾ | 99 |
| Heterogeneous nuclear ribonucleoprotein A2/B1 isoform A2 | 4504447 | HNRNPA2B1 | mRNA processing | T47D | 7–10 | ▾ | 96 |
| ER lipid raft associated 2 isoform 1 | 6005721 | ERLIN2 | Unknown | T47D | 4–7 | ▴ | 95 |
| STARD10 | 31455229 | STARD10 | Unknown | MCF7 | 4–7 | ▾ | 95 |
| S-Adenosylhomocysteine hydrolase | 9951915 | AHCY | Metabolism | T47D | 4–7 | ▴ | 94 |
| Isocitrate dehydrogenase 3 (NAD+), alpha precursor | 5031777 | IDH3A | Metabolism | MDA | 4–7 | ▴ | 91 |
| Ribosomal protein, large, P0 | 12654583 | RPLP0 | Protein biosynthesis | MCF7 | 4–7 | ▾ | 90 |
| Enoyl coenzyme A hydratase, short chain, 1, mitochondrial | 14286220 | ECHS1 | Metabolism | MDA | 4–7 | ▴ | 88 |
| Eukaryotic translation initiation factor 3, subunit 2 beta | 54696064 | EIF3I | Protein biosynthesis | MCF7 | 4–7 | ▾ | 82 |
| Proteasome activator subunit 1 isoform 2§ | 30581141 | PSME1 | Protein degradation | MCF7 | 4–7 | ▾ | 81 |
| Growth inhibiting gene 5 protein | 41350397 | CACYBP | Ubiquitin cycle | MCF7 | 7–10 | ▾ | 81 |
| Mitochondrial aldehyde dehydrogenase 2 precursor | 25777732 | ALDH2 | Metabolism | MDA | 4–7 | ▴ | 80 |
| Pro-alpha 1 (II) collagen | 30097 | COL2A1 | Structural | MDA | 4–7 | ▾ | 75 |
| CNDP dipeptidase 2 (metallopeptidase M20 family), isoform CRA_e | 119586960 | CNDP2 | Peptidolysis | MDA | 4–7 | ▴ | 72 |
| Hypothetical protein LOC80007 | 13376421 | C10orf88 | ATPase activity | MDA | 4–7 | ▴ | 70 |
| Lysyl-tRNA synthetase | 11095909 | KARS | Protein biosynthesis | MCF7 | 4–7 | ▴ | 69 |
Expression levels represent the up- (▴) or down- (▾) regulation of proteins by at least two-fold in the radioresistant derivatives relative to parental cells. Gene ontology information was derived from the GOA database (http://www.ebi.ac.uk/GOA/).
A summary of all confirmed data is shown in Table 5.
*
Proteins for which MS/MS data were obtained.
†
Targets selected for Western blot analysis (Figure 4).
‡
Proteins identified in two separate but closely mapped spots on the same gel.
§
Targets selected for analysis by RT-qPCR.