Skip to main content
. 2009 Nov;11(11):1216–1225. doi: 10.1593/neo.09960

Figure 1.

Figure 1

Effects of HE3235 on C4-2B and LuCaP35V cells in vitro. (A) Chemical structure of HE3235 and AED. (B and C) HE3235 increased AR-mediated transcription in C4-2B cells in vitro as demonstrated by ARE-luc and PSA-promoter-luc reporter assays. C4-2B cells were transfected with ARE-luc (B) or PSA-promoter-luc (C) using Amaxa. After 2 days, the luciferase activity was measured. The luc signal was normalized to renilla luciferase. The results are plotted as fold change over the untreated C4-2B cells. (D and E) Single-cell suspensions of LuCaP 35V cells were prepared by dissociation of subcutaneous tumors. The cells were transfected with ARE-luc (D) or PSA-promoter-luc (E) using Amaxa. Treatment with HE3235 increases AR-mediated transcription in LuCaP35V CaP cells. (F) HE3235 effect on AR-mediated transcription in C4-2B cells. C4-2B cells were grown under standard tissue culture conditions (see Materials and Methods) and treated with HE3235. HE3235 significantly inhibited C4-2B proliferation. RLU indicates relative light units.