Figure 4.

Effects of target treatment of CCL2 on local PC-3^luc tumor growth and macrophage recruitment. (A) Mice received PBS (control) or a combination of anti-human CCL2 CNTO888 (10 mg/kg) and anti-mouse CCL2/JE CNTO1142 (10 mg/kg) from 1 week before PC-3^luc subcutaneous injection (n = 5 per group) to the end of the experiment twice weekly. Tumor growth was recorded as photons per second, and data from the fifth week were plotted. Values represent median (*P < .05). (B) Macrophage recruitment to tumor. Tumors were harvested on the sixth week, and the percentage of Mac-3-positive cells were analyzed as described in Materials and Methods. Values represent mean ± SEM (*P < .05, Student's t test).