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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Virology. 2009 Jul 9;391(2):221–231. doi: 10.1016/j.virol.2009.06.014

FIG. 7.

FIG. 7

Inhibition of RTA binding to ORF57 promoter by K-RBP. (A) The zinc finger domain of K-RBP binds ORF57 promoter. The E. coli expressed His-tagged zinc finger domain, full-length K-RBP and E. coli lysate were resolved on SDS-PAGE, transferred to PVDF membrane and analyzed by Coomassie blue staining (left panel). The second membrane with the same samples was refolded and hybridized to the 57P probe labeled with [α-32P] dATP (right panel). (B) Baculovirus expressed RTA, E. coli expressed K-RBP and [α-32P] dATP labeled 57P probe were used in EMSA. The EMSA was performed as described in Fig. 3C except that different unlabeled competitors and proteins were used as indicated in the figure. Open arrows indicate specific binding of RTA to probes. Filled arrows indicate the binding of K-RBP to probes. Numbers below the bands indicate the relative intensities of the bands measured by Image J.