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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mol Cancer Res. 2009 Jul 7;7(7):1132–1138. doi: 10.1158/1541-7786.MCR-09-0068

Figure 2.

Figure 2

Expression of PIPP interferes with cellular transformation and Akt signaling of class I PI3Ks. (A) Cellular transformation assay. The indicated constructs were transfected in to CEF either with or without PIPP, and cells were overlaid with nutrient agar for 14 days before staining with crystal violet. RCAS(A) and v-jun-transfected CEF are shown as controls. (B) Expression of PIPP does not affect the expression of class I PI3K isoforms. The expression levels of indicated constructs were analyzed with either isoform specific antibodies (p110α, β, δ) or against the VSV tag (p110y). PIPP was detected with HA antibody. (C) PIPP interferes with PI3K/Akt signaling. Downstream targets of PI3K show reduced phosphorylation when cotransfected with PIPP and FoxO protein degradation is attenuated.