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Class III PI3K, hVps34 is not oncogenic in CEF. (A) Focus formation of indicated constructs. RCAS(A) is shown as a negative control, p110α H1047R and p110δ serve as positive controls. (B) Vps34 and Myr-Vps34 do not activate PI3K targets under normal (left panel) or low serum conditions (right panel). Both proteins are VSV-G tagged and detected with a specific antibody against the VSV-G epitope.
