Figure 3. Sulfation of 4-nitrophenol (pmol/min/mg protein) in BeWo cells as a function of 4-nitrophenol concentration.
The figure represents a fit to the substrate inhibition equation (section 2.6). Each assay was repeated at least twice and each point represents the mean ± S.E.M. of three or more determinations. Kinetic parameters were as follows: Km = 0.63 ± 0.21 μM, Ki = 21.2 ± 8.1 μM.