Table I. Primer sequences used for reverse transcribing sulfotransferase mRNA and expected base pair sizes of the reaction products.
Target sequence | Genbank accession ID | Primers (Forward, Reverse) | Expected size (Expected size after digestion) |
---|---|---|---|
SULT1A1 (39-1025) |
L10819 | 5′-ATGGAGCTGATCCAGGACAC-3′ 5′-TGACCTACCGTCCCAGGCCC-3′ |
987 |
SULT1A3 (139-1125) |
L19956 | 5′-ATGGAGCTGATCCAGGACAC-3′ 5′-TGAGCCACTGTGCCTGACTC-3′ |
987 |
SULT1E1 (389-852) |
Y11195 | 5′-ACCTGAACTTCTTCCTGCC-3′ 5′-TCCAGTCTCCTGTAATTCCC-3′ |
464 |
SULT2A1 (21-875) |
U08024 | 5′-ATGTCGGACGATTTCTTATG-3′ 5′-AATCCCATGGGAACAGCTC-3′ |
856 |