Table 1.
Name | Sequence of Primer | |
---|---|---|
1. | fusion- CCH1-Fa−- strategy 1 | gtctagaatggctagccccactcccgcaa |
2. | fusion- CCH1-Rb- strategy 1 | cttctcctttgctagccataccctgttcat |
3. | fusion- CCH1-F- strategy 2 | atggcccactcccgcaaccacagcctcgcaatc |
4. | fusion- CCH1-R- strategy 2 | same as primer #7 |
5. | CMV-F | cgatgtacgggccagatatac |
6. | GFP-R | tagtgcgttcctgtacataaccttc |
7. | CCH1-R | accctgttcattttcaatgccttcttgatctac |
8. | CCH1 seq1 | cagagagaagaggtggatcacggccgacta |
9. | CCH1 seq2 | tactttcttgtttttgctgcgtgcata |
10. | CCH1 seq3 | ggaactgttttgacctttttct |
11. | CCH1 seq4 | acggacgagcatatggatgcatttag |
12. | CCH1 seq5 | tactgttgacgaaagcatttggt |
13. | CCH1 seq6 | atctttttcttctctgggctaccat |
14. | CCH1 seq7 | actattttcaagactatataacga |
15. | CCH1 3521-R | caggcccagagccacttctgtg |
16. | CCH1 5’440-R | tccctgtcgtctgatgatct |
17. | CCH1-CMV-R | ggctgtggttgcgggagtgggccataatttcgataagccagtaagcag |
18. | CCH1-GFP-F | agaaggcattgaaaatgaacagggtatggctagcaaaggagaagaact |
19. | CMV-SacII-F | ccgcgggttaggcgttttgcgctgcttcgcgatgtacg |
20. | PolyA-SacII-R | ccgcggcactacgtgaaccatcaccctaatcaag |
Primers used in the 5’ forward direction are denoted by F.
Primers used in the 3’ reverse direction are denoted by R.