The effects of released-BDNF on cultured neurons. (A) Number of processes per neuron, (B) number of primary processes per neuron (C) Total length of processes, (D) number of branching points per neuron. Values are given as means ± standard deviation. There was no significant difference between substrates (control, polyacrylamide, and PEGDA). The asterisk indicates measures which are significantly different than the corresponding values from 0 ng/mL BDNF. (ANOVA followed by Tukey’s post hoc test, *: P<0.05, **:P<0.01, n=5 for each condition)