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Representative activity recorded at DIV 10 from neural networks cultured (A) on a bare MEA with bath application of 100 ng/mL BDNF, (B) on polyacrylamide hydrogel with 100 ng/mL released-BDNF and (C) on PEGDA hydrogel with 100 ng/mL released-BDNF. (D) Firing frequency plots from 100ng/mL BDNF treated neurons cultured on different substrates (n=3 for each condition).
