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Characterization of released alkaline phosphatase. Kinetics of substrate metabolism.
| control | polyacrylamide | PEGDA | |
|---|---|---|---|
| Vmax (M s-1) | 1.24 × 10-3 | 0.10 × 10-3 | 0.27 × 10-3 |
| KM (mM) | 0.12 | 0.10 | 0.11 |
| kcat (s-1) | 347.2 | 28.0 | 75.6 |
| kcat/KM (M-1s-1) | 2.89 × 106 | 0.28 × 106 | 0.69 × 106 |
