Figure 1. Blastocyst-derived stem cells (FAB-SC).
FAB-SCs were derived as described in the results section. A: Brightfield image of FAB-SCs (Top panel) and mES cells (bottom panel) B: Q-PCR expression analysis of Oct4, Sox2 and Nanog expression on FAB-SC and ES cell as indicated. C: Left panels: Immunofluorescence staining of FAB-SCs for Oct4, Sox2 and Nanog as indicated. Right panels: DAPI nuclear staining. D. Hierarchy clustering of MicroRNA profiles of MEF, mES, EpiSC and FAB-SC cell lines. E. Normalized expression intensity values (scaled median ratio) were obtained from Agilent whole-genome microarrays. Three biological replicates were used for all three cell types.