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. Author manuscript; available in PMC: 2009 Oct 31.
Published in final edited form as: Cell. 2008 Oct 31;135(3):449–461. doi: 10.1016/j.cell.2008.08.035

Figure 3. Growth factor stimulation induces FAB-SC chimera formation and germline contribution.

Figure 3

A Temporal analysis of the integration of GFP-transgenic FAB-SC before and after LIF/BMP4 stimulation into recipient blastocysts. Integration of FAB-SCs and LIF/BMP4 stimulated FAB-SCs was monitored at 1, 3 and 6 hours after injection as indicated. B: Top panel, high contribution chimera derived from LIF/BMP4 stimulated FAB-SC (48 hr stimulation). Middle panel: GFP+ offspring of the FAB-SC chimera, demonstrating germline transmission. Bottom panel: Chimera from clonal FAB-SCs stimulated for 7 days with LIF/BMP4; arrows indicate agouti coat color chimerism.