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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Gastroenterology. 2009 Jun 6;137(3):945–954. doi: 10.1053/j.gastro.2009.05.054

Fig. 6. Inhibitory phosphorylation of the pro-apoptotic protein BAD is enhanced in the Ptk6 −/− intestine after irradiation.

Fig. 6

(A) Immunoblotting was performed using lysates from Ptk6 +/+ and Ptk6 −/− intestines at 0 hours and 6 h post γ-irradiation (γ-IR) with BAD phospho-Ser-112 specific antibodies and antibodies against total BAD. Each lane represents lysate from an independent animal. β-actin expression was examined as a control. (B) There was a significant increase in phospho-BAD levels in the Ptk6 −/− intestine at 6 h following γ-irradiation (*), at which time higher levels of phospho-BAD were detected in Ptk6 −/− than Ptk6 +/+ intestines (**) *P-value = 0.034; **P-value = 0.023.