Figure 6.

Recombination activity of recombinase activating gene 1 (RAG1) mutants. Signal joint (SJ) (a) and coding joint (CJ) (c) assays were performed as described in the Materials and methods, with RAG1 wild type (WT), N265A, H270A, I292A, E294A, S327F or I292A/C317A/S327F (I/C/S) as indicated. Recombination was assessed by polymerase chain reaction (PCR); the average and standard deviation were calculated from three independent trials. The significance of recombination impairment relative to WT was assessed by Student’s t-test (*P < 0·05; ***P ≤ 0·001). (b) Correlation between deficits in recombination and E3 activity was assessed by regression analysis. The data points indicated by small arrows are from previously published work;⁶ remaining data points are from this paper.