Figure 1.
Promoter activity without (wild-type) and with rs884409 SNP. Wild-type or mutant rs884409 SNP luciferase reporter constructs were transiently transfected into Caco-2 cells and relative luciferase activity (RLU) was measured 48 hours later. rs884409 SNP is localized to nucleotide −326 relative to the TSS-1 and had a significant negative association with basal promoter activity (p=0.0043). Data are representative of two independent experiments measured in triplicate, presented as means ±SD and analyzed using 1-way ANOVA and Tukey’s post hoc test. Data is presented as % of Wild-type promoter activity set as 100.
